Henderson Hasselbalch Equation Derivation

This works when water is the solvent and is present in a very large proportion to the h and acid conjugate base.

Henderson hasselbalch equation derivation. The henderson hasselbalch equation also describes the characteristic shape of the titration curve of any weak acid such as acetic acid phosphoric acid or any amino acid. The equation is also useful for estimating the ph of a buffer solution. By its nature it does not take into account the self dissociation of water which becomes increasingly important in dilute solutions. It is widely used to calculate the isoelectric point of proteins point at which protein neither accept nor yield proton.

The henderson hasselbalch equation equation ref hh is an approximation with a certain region of validity. We have already seen the derivation of the henderson hasselbalch equation on this page we discuss some of the applications of the henderson hasselbalch equation and solve some sample problems which one may encounter in research. The reason the henderson hasselbalch equation is an approximation is because it takes water chemistry out of the equation. Lawrence joseph henderson and karl albert hasselbalch.

The henderson hasselbalch equation provides a general solution to the quantitative treatment of acid base equilibrium in a biological system. When concentrations reach somewhere around 10 5 mol l 1 or lower the true ph. How to derive henderson hasselbalch equation. Buffers are the mixture of weak acids and their salts of strong bases or the mixture of weak bases and their salts of strong acids simply buffers are an important concept of acid.

This article explains how to derive the henderson hasselbalch equation. For example the solution may contain. You shouldn t try to apply the approximation for concentrated solutions. Lawrence joseph henderson 1878 1942 was a talented biochemist among many other titles who spent most of his career at harvard.

The henderson hasselbalch equation is widely used by many scientists especially chemists biologists and pharmacists. In chemistry and biochemistry the henderson hasselbalch equation can be used to estimate the ph of a buffer solution the numerical value of the acid dissociation constant k a of the acid is known or assumed the ph is calculated for given values of the concentrations of the acid ha and of a salt ma of its conjugate base a. Example problem applying the henderson hasselbalch equation. The derivation of the henderson hasselbalch equation is therefore significant for any student of pharmaceutical sciences.

The henderson hasselbalch equation equation describes the derivation of ph as a measure of acidity in biological and chemical systems. Henderson hasselbalch equation is a simple expression which relates the ph pka and the buffer action of a weak acid and its conjugate base.